【解答】Essential Biosafety Equipment

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問	BSCs are open workspaces designed to protect laboratory personnelthrough the use of directional air flow and High Efficiency Particulate Air filters.
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✓	╳
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問	Key considerations for selecting appropriate PPE include
	Characteristics of the biological agent
	Type of work being carried out
	Availability of national regulations
✓	All of the above are correct
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問	Essential biosafety equipment do not include
	PPE
	Autoclave
✓	Bench
	Biological safety cabinet
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問	HEPA filter performance of a minimum efficiency of 99.97% should be used
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✓	╳
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問	Things to avoid when using a BSC
	Use open flame
	Rapid arm movements
	Block the grills
✓	All of the above mentioned
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問	Which of the following description of the autoclave is incorrect?
	Do not overload the autoclave
	Use separate autoclaves for ‘clean’ and ‘dirty’ loads
✓	No need to monitor autoclave sterization regularly
	Do not operate overnight
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問	Different types of glove may be needed for different tasks or with other occupational hazards, such as heat or sharps.
✓	〇
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問	Air particles are caught by HEPA through which mechanism?
	Diffusion
	Interception
	Impaction
✓	All of the above are correct
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問	PPE is used only in BSL-2,3 and 4 biological laboratories
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問	Gloves should always be inspected before use to check that they are intact.
✓	〇
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問	When use combinations of PPE needs to consider complementing one another
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問	Which description is incorrect?
	Minimum distance between BSC and door is 1.1 m
✓	Minimum distance between 2 side-by-side BSCs is 1 m
	2 BSCs put in the opposite site along the wall, the minimum space between 2 persons is 3.1 meters
	The airflow curtain that helps provide protection when working in a BSC is fragile
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問	Work surfaces and interior walls need to be disinfected only after each use
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✓	╳
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問	The minimum space between BSC and door is 1.1 meter and the same distance between 2 side-by side BSCs.
✓	〇
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問	Which level of biosafety laboratory required to use PPE?
	BSL-1
	BSL-2
	BSL-3
✓	All of the above are correct
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問	Autoclave sterilization condiction?
✓	121°C for 60 minutes
	100°C for 60 minutes
	85°C for 60 minutes
	121°C for 15 minutes
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問	UV light is not recommended to be used in BSCs
✓	〇
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問	If an autoclave used for sterilize the infectious substances, Its exhaust should be filtered through a filter with membrane size of
	0.1 micron
✓	0.2 micron
	0.3 micron
	0.4 micron
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問	Autoclaving is the most dependable method of sterilizing laboratory equipment and decontaminating biohazard wastes.
✓	〇
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問	Which form of PPE is one of the most critical and easily implemented ?
	Footware
✓	Eye protection
	Lab coat
	Respiratory protection
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問	After moving a BSC within the laboratory, it is not nessersary to certified the BSC.
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問	Which class of BSC can not protect ptoducts?
	Class III
✓	Class I
	Class II A1
	Class II B1
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問	When infectious materials are sterilized, exhaust of an autoclave should be filtered through a filter with 0.2 micron membrane
✓	〇
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問	Which class of BSC do not have HEPA filtered supply air?
	Class II
	ClassIII
✓	Class I
	Class II B1
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問	The proper type of PPE must be chosen and personnel must be trained in its proper use and know how to detect any faults
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問	Which one is not a key consideration for selecting PPE?
	Volumes and concentrations of the biological agent
✓	Color
	Other PPE being worn
	Individual needs of the laboratory personnel
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問	Which of the following descriptions of the functions of a biological safety cabinet is incorrect?
	Air in the Class I, Class II B2, and Class III BSC is 100% exhausted
	There are 3 classes of BSC
✓	None of BSC can be applied to handle volatile toxic chemicals
	Class II A1 and A2 BSCs can be a thimble connected
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問	The laboratory director should ensure that the adequate equipment is provided and used properly.
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問	Which PPE is not a core requirement?
✓	Respiratory protection
	Gloves
	Lab coat
	Footware
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問	All classes of BSC can protect personnel, enviroment and product
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✓	╳

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