【解答】Essential Biosafety Equipment

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BSCs are open workspaces designed to protect laboratory personnelthrough the use of directional air flow and High Efficiency Particulate Air filters.
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Key considerations for selecting appropriate PPE include
Characteristics of the biological agent
Type of work being carried out
Availability of national regulations
All of the above are correct
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Essential biosafety equipment do not include
PPE
Autoclave
Bench
Biological safety cabinet
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HEPA filter performance of a minimum efficiency of 99.97% should be used
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Things to avoid when using a BSC
Use open flame
Rapid arm movements
Block the grills
All of the above mentioned
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Which of the following description of the autoclave is incorrect?
Do not overload the autoclave
Use separate autoclaves for ‘clean’ and ‘dirty’ loads
No need to monitor autoclave sterization regularly
Do not operate overnight
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Different types of glove may be needed for different tasks or with other occupational hazards, such as heat or sharps.
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Air particles are caught by HEPA through which mechanism?
Diffusion
Interception
Impaction
All of the above are correct
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PPE is used only in BSL-2,3 and 4 biological laboratories
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Gloves should always be inspected before use to check that they are intact.
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When use combinations of PPE needs to consider complementing one another
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Which description is incorrect?
Minimum distance between BSC and door is 1.1 m
Minimum distance between 2 side-by-side BSCs is 1 m
2 BSCs put in the opposite site along the wall, the minimum space between 2 persons is 3.1 meters
The airflow curtain that helps provide protection when working in a BSC is fragile
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Work surfaces and interior walls need to be disinfected only after each use
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The minimum space between BSC and door is 1.1 meter and the same distance between 2 side-by side BSCs.
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Which level of biosafety laboratory required to use PPE?
BSL-1
BSL-2
BSL-3
All of the above are correct
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Autoclave sterilization condiction?
121°C for 60 minutes
100°C for 60 minutes
85°C for 60 minutes
121°C for 15 minutes
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UV light is not recommended to be used in BSCs
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If an autoclave used for sterilize the infectious substances, Its exhaust should be filtered through a filter with membrane size of
0.1 micron
0.2 micron
0.3 micron
0.4 micron
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Autoclaving is the most dependable method of sterilizing laboratory equipment and decontaminating biohazard wastes.
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Which form of PPE is one of the most critical and easily implemented ?
Footware
Eye protection
Lab coat
Respiratory protection
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After moving a BSC within the laboratory, it is not nessersary to certified the BSC.
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Which class of BSC can not protect ptoducts?
Class III
Class I
Class II A1
Class II B1
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When infectious materials are sterilized, exhaust of an autoclave should be filtered through a filter with 0.2 micron membrane
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Which class of BSC do not have HEPA filtered supply air?
Class II
ClassIII
Class I
Class II B1
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The proper type of PPE must be chosen and personnel must be trained in its proper use and know how to detect any faults
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Which one is not a key consideration for selecting PPE?
Volumes and concentrations of the biological agent
Color
Other PPE being worn
Individual needs of the laboratory personnel
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Which of the following descriptions of the functions of a biological safety cabinet is incorrect?
Air in the Class I, Class II B2, and Class III BSC is 100% exhausted
There are 3 classes of BSC
None of BSC can be applied to handle volatile toxic chemicals
Class II A1 and A2 BSCs can be a thimble connected
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The laboratory director should ensure that the adequate equipment is provided and used properly.
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Which PPE is not a core requirement?
Respiratory protection
Gloves
Lab coat
Footware
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All classes of BSC can protect personnel, enviroment and product
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